Pure titanium dioxide (TiO_2) is a semiconductor, which means its electrical conductivity is very low. The properties of titanium dioxide such as electrical conductivity, absorption coefficient, refractive index, concentration, temperature and pH-values were measured based on diffusion techniques. Absorption coefficient decreases with increasing the thickness of the titanium dioxide. As increasing the thickness of titanium dioxide decreases the intensity of gamma radiation. The absorption coefficient depend upon the absorbing material and the intensity of the radiation. Higher the absorption coefficient was enviable where the material covers soft tissue. A small absorption coefficient specify that the material was relatively transparent. The pH-values of sample does not depend on concentration of the titanium dioxide. It indicate that antacid potential present, which will be caused by biological infective resistance to human beings. The temperature of the samples increases conductivity also increases gradually. The concentration of the sample increases conductivity also decreases. The diffusion technique enables the measurement of the electrical properties of liquids. This biological effect was more significant for pharmacitical applications. By varying the film deposition and annealing conditions titanium dioxide refractive index changes with wavelength. The variations in refractive index that reduces the refractive of a surface treated with an anti-reflective coating.