Silybum marianum (L.) is a medicinal plant containing Silymarin – a complex of flavonolignans: Silybin A, Silybin B, Isosilybin A, Isosilybin B, Silychristin A, Silychristin B, Silydianin, Silandrin, Isosilandrin, Silychermin, Neosilychermin, Siliamandine and Siliymonine. Silymarin possesses antioxidant, anticarcinogenic, anti-inflammatory, antifibrotic, hepatoprotective, immunomodulatory and neuroprotective activity and is applied in liver cirrhosis, acute and chronic viral hepatitis. Hepatoprotective action is a result of antioxidant, anti-lipid peroxidative activity and of reduction of glutathione oxidation.
Objective: The aim of cuttent study was to compare the different modificated DPPH methods for determination of free radical scavenging activity of Silybum marianum L. extracts.
Methods: A literature survey has been applied by using of different data bases as Scopus and Medline, Google Scholar, PubMed.
Results:
For the estimation of free radical scavenging activity of Silybum marianum L. extract, the modifications of DPPH method comprise the changing of :
1) reaction time (from 30 min. to 90 min.)
2) solvent for DPPH (methanol, ethanol, butanol)
3) concentration of DPPH (from 0.1 M to 0.006 mM, 0.15 mM, 0.2 mM, 0.3 mM, 1 mM)
4) proportion between volumes of DPPH and plant extracts
5) absorption maximum: from λ = 517 nm to λ = 515 nm or λ = 520 nm.
Conclusion:
For different modifications of DPPH method for the assessment of free radical scavenging activity of Silybum marianum L. extract, the following most important conclusions can be summarized:
I) the most often applied modifications are in connection with the changing of:
1) concentration of DPPH solution: 0.1 mM to 0.006 mM, 0.15 mM, 0.2 mM, 0.3 mM, 1 mM,
2) proportion between volumes of DPPH and plant extract
II) the most often used conditions for DPPH method are:
1) 30 min. reaction time between DPPH solution and plant extracts
2) application of methanol as solvent
3) absorption maximum: λ = 517 nm.
Impact Factor
2022: 6.012
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