Comparative evaluation of four different remineralizing agents on the bleached enamel surface by using sem with edx: an in vitro study

To evaluate the effect of 22% carbamide peroxide (CP) on the mineral content of enamel and to evaluate the remineralization of bleached enamel after treatment with four different remineralizing agents.
Materials and methods: About 40 extracted human maxillary incisors were cleaned using ultrasonic scaler. Analyzed using scanning electron microscopy with EDX (Energy Dispersive X ray Spectroscopy) to determine the initial mineral content. 22% CP was applied to the labial surfaces 8 h/day for 2-week. Then, samples were washed and stored in artificial saliva. Post-bleaching mineral content is analyzed by using scanning electron microscopy with EDX. The samples were randomly divided into four groups. Group A: GC tooth mousse plus. Group B: Toothmin, Group C: 6.5% (w/v) GSE solution. Group D: 6.5% (w/v) Wheat Grass solution. Remineralizing agents were gently agitated on the bleached enamel with an applicator tip for 10-min and cleaned thoroughly using deionized water. The mineral content of bleached enamel after the remineralization regimen was assessed using EDX. Statistical analysis was done using one-way ANOVA.
Results: Bleaching with 22% CP significantly decreased the mineral content of the enamel. Group B Toothmin showed the highest remineralization than Group A, C and D but was not statistically significant.