Objectives of Study: This study aimed to compare the Rapid card test with Enzyme linked Immunosorbent assay and to detect the seroprevalence of Dengue infection in Greater noida.
Methods: A Cross-Sectional study was carried out in the Department of Microbiology, Central Laboratory, School of Medical Sciences & Research, Sharda University, Greater Noida from 1st Novemenber 2020 to 31st October 2021. A total of 4516 samples were received in serology laboratory for NS1 Ag test. 386 were randomly selected to be included in the study with 193 positive samples and 193 negative samples for dengue identification by Immuno-chromatography test (ICT) (SD Bioline Dengue Duo) based RDT kit which detects NS1 antigen. The samples tested by ICTs subjected to ELISA (Erba Mannheim) tests for Confirmation NS1 antigen.
Results: The Rapid Dengue Test showed a sensitivity, specificity, positive predictive value and negative predictive value were 91.66%, 95.45%, 95.65% and 91.30% respectively for NS1 Ag detection. Seroprevalence of Dengue infection was found to be 30.22%.
Conclusion: Good sensitivity and specificity of Immuno-chromatography test (ICT) for early detection of dengue NS1 Ag was observed. We concluded that, an ideal Rapid test is a boon in time –saving can be easily performed by any trained health care worker at any time of need. Hence, Dengue NS1 Ag screening can be preferably done by Rapid Card test followed by a supplemental ELISA and polymerase chain reaction for further confirmation.
Impact Factor
2022: 6.012
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