A comparative study to evaluate the antifungal efficacy of nano graphene oxide material and clotrimazole incorporated in soft liners in complete dentures- an in vitro study

Title: A comparative study to evaluate the antifungal efficacy of nano graphene oxide material and clotrimazole incorporated in soft liners in complete dentures- an in vitro study. Methods: 30 samples were prepared out of which 15 were coated with nano graphene oxide of 20 nm size and 5% solution combined with chloroform. The remaining 15 discs were incorporated with Clotrimazole. The above discs were placed on the flat bottom of well cell culture plates. Hundred microlitre of initial suspension in sabouraud’s broth was inoculated to each well and incubated at 37 degree celsius. After incubation for 24 hours and 72 hours for extended contact period, suspension was withdrawn. Viable cells in the suspension were determined by using the spread plate method at the level of detection. The data obtained was subjected to statistical analysis. Results: Results of the study showed that, 1% Clotrimazole showed a larger zone of inhibition when compared to that of Graphene oxide combined with Chloroform. Thus, confirming the antifungal efficacy of soft liners containing graphene oxide and clotrimazole against candida species. Conclusion: Antifungal efficacy of 1% Clotrimazole was found to be better than Graphene oxide with Chloroform against Candida albicans.