Objective: To do detailed microscopic study of the morphology of the reproductive tract of the male Necator americanus (hookworm) by retrieving out while doing upper gastro-intestinal endoscopy. Methods: All patients who had undergone upper gastro-intestinal endoscopy for a period of 2 years (2017 and 2018) were examined for the presence of parasitic worms. Results: Out of these patients who had undergone upper gastro-intestinal endoscopy, 20 patients were found to have hookworms in duodenum. The head end and mouth of the hookworm is bent into a curved shape giving it a hook like appearance and hence it is identified as hookworm. Conclusion: Hence upper gastro-intestinal endoscopy is a very useful investigation to diagnose hookworm infection.