Association of gabrb3 (rs2059574) and gad67 (rs2177433) gene polymorphisms with nonsyndromic cleft lip and palate in south indian population using dna sequencing

Background: Non-syndromic cleft lip with or without cleft palate (CL/CP) is a common congenital facial malformation without any other structural or developmental abnormalities.
Objectives: To evaluate the association of GABRB3 gene variant rs2059574 and GAD67 gene variant rs2177433 gene polymorphisms with non syndromic cleft lip and palate in South Indian population.
Methods: DNA samples of 25 subjects with non syndromic cleft lip and palate and 25 unrelated controls, collected from the department were used for the study. Group A: - DNA samples of 25 subjects with Nonsyndromic cleft lip and palate (P1-P25)Group B: - DNA samples of 25 unrelated controls (C1- C25).The extracted DNA samples were subjected to Polymerase chain reaction and later these amplified products were subjected to DNA sequencing. Results were documented in the form of electropherograms.
Results: The results indicated that there is a strong association between the presence of GABRB3gene variant rs2059574 with the incidence of Nonsyndromic cleft lip and palate. This study suggests that the likelihood of Non syndromic cleft lip and palate is higher in subjects having TT (p<0.001) genotype for GABRB3 rs2059574.
Conclusion: We can conclude that GABRB3gene variant rs2059574 can be considered as genetic markers for Non syndromic cleft lip and palate for our population.