Standardization of herbal and herbo-mineral drugs refers to confirmation of identify and determination of its quality, purity and detection of adulteration by various parameters like morphological, microscopical, physical, chemical and biological observations. As per Pharmacopeial Laboratory of Indian Medicine (PLIM) guidelines there are some parameters to follow the standardization techniques to improve the efficacy of Siddha drugs. As a consequence of focusing upon the current need of drug standardization the present work was undertaken to standardize the traditional Siddha formulation mātuḷainey as per PLIM guidelines given forney.