Antagonist activity of streptomyces sampsonii mn 93/2 and research on jasmonic acid

Author: 
Otgonjargal Khuredagva., Tseweendari Chuluunbaatar., Temuujin Janchiv, Gantuya Myagmarsaikhan and Esentaish Gantsolmon

As a result of isolation of Streptomyces actinomycetes, which are antagonists of some plant fungal pathogensfrom the soil using Humic acid, vitamin agar and selective nutrient medium,a pure culture similar to Streptomyces in terms of morphology was isolated and it was the closest to Streptomyces sampsonii when determining species affinity through 16SrRNA sequential analysis.
Upon determination of antagonist activity of Streptomyces sampsonii MN93/2 culture by double culture method, it was determined to have antagonist activity, creating 15.5 mm inhibition zone forCladosporium fulvum and 19 mm inhibition zone for Alternaria alternata pathogens.
Tests of S.sampsonii MN93/2 under greenhouse conditions, neutralized the course of tomato alternariosis and showed 72.1% biological activity in 21 days. Streptomyces sampsonii MN93/2 contains 0.00679 mg /kg of jasmonic acid in plants treated with 107cells/ml and 0.0267 mg / kg in plants treated with 108cells/ml.

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DOI: 
http://dx.doi.org/10.24327/ijcar.2020.23446.4642
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Volume9