Soil is a habitat of large number of micro- organisms viz bacteria, fungi, actinomycetes, algae and protozoa etc. constituting biotic environment of soil micro-ecosystems. These micro-organisms play important role in numerous physiological activities including biogeochemical cycle. For this microbial community forms associations with other organisms such as saprophytic, parasitic and symbiotic associations. Some of the soil inhabiting rhizospheric fungi have been found as growth promoting while some are pathogenic. Several soil fungi have been found to have symbiotic association with roots of higher plants which are called as mycorrhizal associations. Mycorrhizal associations in plants are of two type ectomycorrhiza or extra matrical spore and endomycorrhiza. Extra matrical spores of mycorrhiza are quite common and can survive in environment of low fertility, drought, disease and temperature extremes, where alone they fail to survive. Present work has been carried out to isolate and identify mycorrhizal spores growing symbiotically with roots of trees in Babina forest range of Jhansi district of Uttar Pradesh. For this surveys have been carried and soil samples have been collected. Using wet sieving method spores have been isolated and identified on the basis of shape, size and colour. Spores belongs to genera are Acaulospora, Glomus, Enthrophospora, Gigaspora and Scutellospora out in different seasons such as winter summer and rainy from December 2017 to November 2018.