An electrochemical method for the quantitative detection of acetaminophen at a glassy carbon modified with Bismuth composites (Bi/GCE) is presented for the first time. In this research, Bismuth composite were effectively prepared by ultrasonic method, and a best electrochemical sensor-Bi/GCE was formed for analysis of Acetaminophen (AC). Scanning electron microscopy (SEM), transmission electron microscopy (TEM) were used to define the morphology of modified electrode. The electrochemical behavior of AC on the modified electrode was studied using cyclic voltammetry (CV) and differential pulse method (DPV) in pH 7.0 phosphate buffer. The results presented that in the neutral environment, AC had a clear electro catalytic effect on the modified electrode, and the reaction of AC on the modified electrode was controlled by adsorption. Sensor is used to investigate the utility of the proposed method for the determination of Acetaminophen in real samples.