Dietary supplements are consumed in large quantities among the youth now a days. The purpose of this study was to assess the knowledge, attitude and practice (KAP) regarding supplements and its use among state and national players. The cross-sectional study (knowledge, attitude and practice) was conducted at Netaji Subhas National Institute of Sports and Punjabi University, Patiala.Total198 Players were tested which comprised of 70 male and 29 female players in each category. The tool used to collect the data was semi-structured questionnaire which was developed to assess KAP regarding the supplements. The mean height of the subjects was 169.4cm±3.0 cm and 175cm±12.3cm for state players &national players respectively. The mean weight of the state players was 70.4kg±11.9kg and 73.2Kg±13.6Kg for National Players. The result indicates that 90% players knew about supplements but they did not know how to use them. About 86% of the subjects said that consuming supplements was helpful and 11% said that it was not helpful. Despite of awareness and non-awareness, subjects have variants of thoughts regarding supplements. No statistically significant difference was found in both the groups with reference to use of dietary supplements. The total KAP score depicts that majority of players had knowledge but need more guidance and counselling regarding the use of dietary supplements.