Gold nanoclusters synthesized using soybean trypsin inhibitor (sti) with excellent brightness and application for the detection of hg2+

Author: 
Helena T.N.N Amunyela, Yuliang Cheng, Yunfei Xie, Hang Yu, Weirong Yao, Yahui Guo and He Qian

Lately, the applications of metal nanomaterials are continuously increasing in numerous areas. However, during production, some of these methods can be hazardous to the environment, plants, and animals. Thus, there is a need for environmental friendly of metal nanomaterials during production. In this research, gold nanoclusters (Au NCs) were synthesized a new natural enzyme protein soybean trypsin inhibitor (STI) as protecting ligand. The results showed that the newly synthesized STI-Au NCs exhibited a very strong red fluorescence emission at 689 nm and excitation 513 nm. Optimum favourable conditions such as STI concentrations, buffers and time for the synthesis of STI-Au NCs were studied for the brightest fluorescence emission. This newly synthesized STI-Au NCs was used for the detection of mercury (Hg2+). The linear ship range for the Hg2+ ions is from 0.01 - 0.05 µM with a detection limit of 0.71 nM. Additionally, this fluorescence STI-Au NCs probe can be easily synthesized and have generated a high fluorescence, hence, broadening its application in various fields of nanoscience.

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DOI: 
http://dx.doi.org/10.24327/ijcar.2019.17755.3377
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Volume8