Algae are become one of the most auspicious long-term sources for food, oil, fuel and other co-products. Algae produced around 15,000 novel compounds and they were resolved through chemically. Most of the microalgae generated significant products such as fatty acids, antioxidants, polymers, sterols, carotenoids, and enzymes. The aim of the study is the growth parameter analysis, especially growth media, pH and temperature. These three parameters are major role for cultivation of micro algae. Different types of medium were used BBM, M-8 and BG11 for Scenedesmus dimorphus, Coelastrum microporum and Selenastrum bibraianum, different pH such as 4, 4.5, 5 up to 8.5 with 0.5 interval gap and different temperatures such as 20°C to 40°C with the interval of 5°C. BBM showed significant growth with 212, 262, and 342 mg/L/day, pH 7.5, 7.5 and 6.5 with 258, 293 and 432 mg/L/day and 300C with 192, 255, 316 mg/L/day.