Objectives: To assess the prevalence of chronic fatigue syndrome among teaching professionals. Method: Descriptive survey method was adopted. The study was conducted at selected 18 schools in chennai. 680 samples who fulfilled the inclusive criteria were included in this study using non probability convenient sampling technique. Center for Disease Classification short form of symptom inventory for Chronic Fatigue Syndrome was used for data collection. Results: The percentage distribution of Chronic Fatigue Syndrome among teaching professionals showed 17.78% CFS cases and 82.22% non CFS cases. The reliability of the tool was measured by test and retest method. Conclusion: This report can provide essential information to build a strategy for development of therapeutics against chronic fatigue and chronic fatigue syndrome.