The study documented a comparative evaluation of antioxidant potential of crude extract of fungal endophyte in different growth media and extraction solvents through free radical scavenging assay. Endophytic fungal isolate of Aspergillus sydowii was obtained from the leaf of Ananus comosus L. Three different fungal growth media were used. Two different solvents were used for extraction of secondary metabolite from mycelial mat and culture filtrate. Differences in antioxidant activity in respect of growth media and extraction solvents were observed. Ethyl acetate extract of mycelial mat harvested from CYB medium found to have highest antioxidant activity.
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