The current research included greenhouse studies on the evaluate efficacy of four compatible Trichoderma spp. (T.4, T.6, T.7 and T.9), in combination with each other in controlling five phytopathogenic fungi; (Fusarium oxysporum R6, F.solani R11, Curvularia lunata R7, Alternaria tenuissima R23 and Thanatephorus cucumeris R12) by using local rice variety cv. AL-Baraka. Four Trichoderma isolates consortium (T.4+T.7, T.4+T.9, T.6+T.7 and T. 4679), were selected based on pre-screened with pathogens via the dual culture technique to determine its compatible activity against pathogens under laboratory conditions. The experiment was carried out under uncontrolled condition in greenhouse with non-sterilized field soil, and thirteen parameters were evaluated. The results showed that rice plant inoculated with Trichoderma spp. triggered the highest level of chitinase, peroxidase, PAL (Phenyl alanine-ammonialyase) and chlorophyll content in plants, two months post planting. Significant differences were observed in all treatments compared to untreated control. In addition, the results showed the significant interaction between compatible Trichoderma spp. on growth parameters of rice plant, fresh weight of shoot and root, dry weight of shoot, root and panicle, shoot and root length. Trichoderma T.4679 exhibited most compatible and greater efficiency of reducing disease severity when treated with A. tenuissima R23 and T. cucumeris R12 (8.883, and 11.553%, respectively), as compared with control (pathogens alone) which gave significant increase (p<0. 05) of (70. to 88.867 %, respectively) 120 days after transplanting. As a consequence of dual inoculation, the greenhouse experiment determined Trichoderma T.4679 as an effective component in an integrated pest/pathogen management (IPM) program to control rice disease.
Impact Factor
2022: 6.012
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