The aim of this study was to investigate the role conflict on the basis of occupational stress and gender among secondary school teachers. Role conflict was treated as dependent variable whereas occupational stress (more and less) and gender (male and female) were treated as independent variables. Descriptive survey method was employed for the present study. A sample of 350 teachers was taken using multi-stage random sampling technique. Teachers Role Conflict Scale by Gupta and Nain (2016) and Teachers Occupational Stress Scale by Jamal and Raheem (2012) were used to collect the data. Two-Way ANOVA with 2x2 factorial design was used to analyze the data. Levene’s Test of Homogeneity of Variance was also applied to test the assumptions of homogeneity of variance for ANOVA. There was found a significant main effect of occupational stress; and gender on role conflict of secondary school teachers. A significant interaction effect of occupational stress and gender was reported on role conflict of secondary school teachers.The findings of the present study suggested that the employers and administrators should supervise the institutions in such a way to keep the level of role conflict and occupational stress among teachers at minimum so that maximum productivity and effectiveness can be obtained from the teachers.