Alterations of hematological parameters under chlorpyrifos intoxication in mice

Hematology is defined as the branch of biology, which deals with the morphology of blood and blood forming organs. Chlorpyrifos is one of the most widely used Organophosphates (OP) insecticides. Anemia and alteration in other hematological parameters have been recorded following repeated Chlorpyrifos exposure.In the present study Healthy adult mice of same age (100±10 days) and weight (75±10 g) were divided into four groups having ten animals each. The second, third and fourth groups of animals were termed as experimental animals. To the animals of second group single dose of pesticide (i.e. on 1st day) was administered orally by gavage method. To the third group of animals double doses were given i.e. on 1st and 3rd day. Similarly multiple doses i.e., 1st, 3rd, 5th and 7th day were given to the fourth group of animals. The first group of animals was considered as controls. In the present investigation the effect of chlorpyrifos, on the blood is determined in Mice. Oral administration of chlorpyrifos produced statistically significant (P<0.01) decrease in RBC, Hb, PCV but WBC count shows increased level in single, double and multiple doses respectively. The Mean corpuscular volume (MCV), Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Haemoglobin Concentration (MCHC) are dependent on the RBC count, MCV, MCH and MCHC showed statistically significant (P<0.01) decrease in single, double and multiple dose of chlorpyrifos intoxication in Mice.