Isolation, identification of marine fungi with antibacterial activity

The marine environment is a tremendous source of natural products. Marine microorganisms have become an important source of pharmacologically active metabolites. Marine fungi are the potential and promising sources for biologically active secondary metabolite production. Secondary metabolites are the chemical compounds that are produced during the stationary phase of the organism. Many years of study revealed that fungi are excellent sources for novel bioactive secondary metabolites. In the present study fungi were isolated from marine soil samples and serial dilution method was performed to isolate single colonies. Later microscopic and macroscopic observations were done to identify the organism. Organisms are inoculated into medium for production of secondary metabolites. One week later ethyl acetate is added to the broth in 1:1 ratio and kept for incubation in orbital shaker at 170rpm. Solvent is separated by separating funnel and is extracted by rotavapour to collect the crude extract.