Bacterial wilt caused by Ralstonia solanacearum is one of the major constraints for the cultivation of tomato in Kerala and cent per cent crop loss has been estimated in susceptible varieties. Biocontrol using endophytic microorganisms is one of alternative control methods to support agriculture sustainability. The objective of these experiments are to isolate endophytes from root and stem of healthy tomato plants from 16 locations of north, central and south Kerala and to estimate their biocontrol potential against the bacterial wilt pathogen. Among 154 endophytic isolates obtained, 12 out of 79 bacteria, 16 out of 68 fungi, and four out of seven actinomycetes were antagonistic toR. solanacearumin in vitro. Among them, five bacteria, eight fungi, and two actinomycetes were promising in planta.