Antimicrobial and antioxidant activity of butea monosperma linn.in vivo and in vitro

The present research deals with antimicrobial and antioxidant activity in parts and callus cultures of Butea monosperma. The ethanolic extracts exhibited antimicrobial activities with zones of inhibition ranging from 4 to 16 to mm. All the extracts exhibited appreciable activity against all the clinically important bacterial and fungal species Maximum Inhibition zone (14mm) was observed in callus (14mm) against E.coli and minimum in seeds (4mm) against Bacillus subtilis. In case of fungus,maximum activity was observed in callus (16mm) against A. niger and minimum against T. reesei The antimicrobial activity of the extract was compared with the standard drugs. The ability of the crude extracts of B. to inhibit the growth of various bacteria and fungi showed its broad spectrum antimicrobial potential, which may be employed in the management of microbial infections. Further antioxidant activity was done using FRAP, Lipid Peroxidation Assay and Peroxidase assay. In the present investigation maximum (240 µm/l/gdw) antioxidant activity was found in methanol extract of flowers and minimum in hexane fraction of Seeds (34 µm/l/g dw) using FRAP Assay.Here maximum (61.3 µm/l/g dw) LPO activity was observed in callus cultures and minimum (40.3 µm/l/g dw) in flowers.Peroxidase assay showed maximum (8.18 µm/l/g dw) antioxidant activity in callus cultures and minimum (2.87µm/l/g dw ) in seeds of B.monosperma. Hence this study offers a base of using as herbal alternative for the synthesis of antimicrobial and antioxidant activity.