Assessment of internalization of iron in molday ion rhodamine b labelled ovine adipose derived mesenchymal stem cells

Ovine adipose derived mesenchymal stem cells (oADMSCs) are isolated, expanded and passaged up to passage 6. In-vitro cultured cells at passage 4, 5 and 6 were used for Molday Ion Rhodamine-B (MIRB) (25µl/ml concentration) labelling and the internalization of iron was assessed 72 hrs after labelling. Quantification was done by colorimeteric iron assay using 10M HCl and 5 per cent Pottasium Ferrocyanide. In labelled oADMSCs, the quantity of iron is 17-18.2pg of Fe/oADMSCs. In this concentration viability and differentiation potential of labelled oADMSCs was not affected. The results of this study infer that MIRB can be used for labeling the oADMSCs for tracking Mesenchymal stem cells without affecting its viability.