As, there has been a paucity in detecting such virulence markers among dental isolates of Enterococcus faecalis in Indian context, we have taken this objective to find out such factors to rule out the relationship between the pathogen and the disease establishment. It indicates that these genes may even play a cardinal role in causing dental caries by E. faecalis.A sum of 20 dental caries enterococcal isolates were detected for enhanced expression of pheromones and quorum sensing system genes by PCR.4/20 (20%) were showed positive for enhanced expression of pheromone (eep) and 2/20 (10%) were found to have quorum sensing system (fsr) genes by PCR.It indicates that these genes may even play a cardinal role in causing dental caries by E. faecalis. However, it is important to include more number of isolates to validate the result.