The purpose of this study is to find out the efficiency of high intensity interval training on malondialdehyde (MDA) and aerobic capacity of male physical education students. To achieve the purpose thirty (30) male physical education students were selected from Swami Vivekanand Subharti University, Department of Physical Education, Meerut, Utter Pradesh. These subjects were tested on malondialdehyde (MDA) and aerobic capacity before and after eight weeks of high intensity interval training (HIIT). The mean maximal aerobic speed 4.21 m/s was used as a criterion velocity to set running paces for highintensity interval training. Statistical technique used in the present study was ANCOVA for malondialdehyde and aerobic capacity. The result of the study revealed that eight weeks of high intensity interval training resulted in 8.20% of improvement in aerobic capacity (F (1,27) =108.04, p < 0.05), however lipid peroxidation (F = 5.47, p < 0.05) was reduced by 26.53%. It is concluded that high intensity interval training for eight weeks resulted in improvement of aerobic capacity and reduction in lipid peroxidation for selected male physical education students