Introduction- Hospital-acquired infection remains the hospital’s single most serious concern. CSSD ensures availability of reliably sterilized articles at the required time and place in the hospital as economically as possible, having regard to the need to conserve the time of users. Aim and objectives- This study was conducted with the aim of studying functioning, workflow and process of sterilization employed in CSSD of a teaching tertiary care institution of Haryana. Material and methods- This observational study was conducted in CSSD of a tertiary care teaching hospital of Haryana over a period of three months i.e. February 2018 to April 2018 using an observation checklist. Prior permission for the study was obtained from appropriate authorities. Observations- CSSD is centrally located in the hospital premises and has 11 staff members. Workflow ranges from receipt, cleaning, sorting, packing, sterilization and storage of articles. Steam sterilization and gas sterilization is performed by a total of seven autoclaves and 2 ETO machines. Conclusion- CSSD of the institution is contributing its part towards reduction of nosocomial infections and quality of standards for sterilization is well maintained. Staff must be provided refresher training to update them.