Moringa oleifera is a common plant known for various medicinal properties. The research work was conducted to investigate the in vivo antibacterial activity of aqueous, ethanolic and acetonic extract of Moringa oleifera flowers. In-vitro antibacterial activity was performed by well diffusion method in agar medium. Alcoholic extract of flowers has shown effective antibacterial activity in assay techniques. The results obtained in the present study indicate that the study indicate that the flowers of Moringa oleifera are a potential source of natural antibacterial activity.