The objective of our study is to examine the immunostimulatory activity of leaves aqueous extract of Jasminum auriculatum against specific hepatitis B vaccine antigen on human whole blood using flow cytometer. Human whole blood were treated with variable doses of aqueous extract (0.5 – 30 mg/ml) of Jasminum auriculatum and evaluated the lymphocytes, monocytes and granulocytes count and observed the forward (shape and size) and side scatter (granularity of the cell) which is evaluated through flow cytometer and also determined its hemolytic activity. The results showed that the leaves aqueous extract of Jasminum auriculatum showed increased in the number of monocytes and granulocytes count which is evidenced through the retain and decline in the level of forward and side scatter on human whole blood. At high doses of aqueous extract i.e. 30 mg/ml showed hemolytic activity as compared to control. The results showed that the aqueous extract (leaves) of Jasminum auriculatum showed immunostimulatory activity against specific vaccine antigen.
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