Ethnobotanical survey was carried on the medicinal plants used by the Malayali tribes of Jambuthu Malai, Salem District, Tamil Nadu for their veterinary healthcare practices during June 2015 to Aug 2016. Ethnobotanical data was gathered through personal interviews among the traditional healers, farmers and elders of the tribal communities through periodical field visits in the study area. A total of 48 species of ethno veterinary medicinal plants belong to 30 families were recorded with the help of nine ethno veterinary traditional healers. Among the plants parts used, leaves are commonly used for the ethno veterinary medicinal preparations. This information suggests the documentation of the medicinal plants and associated indigenous knowledge can be used for conservation and sustainable use of medicinal plants in the area and for validation of these plant preparations for veterinary treatment.