The present study explained the Photocatalytic and antibacterial activity of mesoporous Mn-S co-doped TiO2 nano material synthesized by using sol-gel method. As prepared samples were characterized by XRD, SEM-EDX, FT-IR, UV-Vis-DRS, TEM and BET. These Characterization and experimental results revealed that there is a formation of anatase phase, presence of co-doped ions and their oxidation states, spherical with smooth surface morphology, frequency shift of Ti-O-Ti in co-doped TiO2 due to substitutional doping of Mn and S into TiO2 lattice, decreased band gap energy and particle size, increased in surface area. The efficiency of photocatalytic and antibacterial activity was evaluated by degradation of Xylenol orange (XO) and Sphingomonas paucimobilis (MTCC-6363) respectively. The complete degradation of XO was achieved in 120 min.