Development of simple methods for the preparation of nanosized metal particles has attracted significant attention because of their future applications due to unusual size-dependent antibacterial properties. Copper (Cu), nanoparticles were prepared by a simple chemical method; The formation of nanoparticles was first screened by using UV-vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The morphology of the synthesized CuNPs was determined using TEM, which indicated that the CuNPs were spherical in shape. Antibacterial activity of the synthesized nanoparticles was tested against bacterial pathogens such as Eschirichia coli, Staphylococcus auerues, Pseudomonas aeruginosa, Proteus vulgaris and Salmonella typhimurium. The synthesized Copper nanoparticles (CuNps) showed effective antibacterial activity against bacterial pathogens. The study supports that CuNPs have good antibacterial activity against gram-negative and gram-positive bacteria.