“There is nothing as powerful as a mother’s love and nothing as healing as a child soul. Objectives-To assess the existing knowledge regarding child rights among mothers of preschool children. To evaluate the effectiveness of Self-instructional module on knowledge regarding child rights among mothers of preschool children. To find out the association between post-test knowledge scores and the selected demographic variables of mothers of preschool children. 60 sample taken from community area of Akola district by survey method. Results-The overall pre-test and post-test percentage of knowledge score was 41.67% and 82.76% respectively. The overall mean of the pre-test score was 12.5 After the intervention the overall mean of the post-test score was 24.83. The calculated “t” value was 25.68, shows that there is a significant increase in the knowledge after administration of self-instructional module. The chi-square value shows that there is significant association between the age, residence, religion, educational status, number of children and post-test knowledge score among mother of preschool children regarding knowledge on child rights. Conclusion-The mothers of preschool children knowledge had enhanced after the administration of self Instructional Module regarding child rights. Hence Education about knowledge on child rights will promote the knowledge of mothers of preschool children.