Analysis of drug utilized the organic solvent which are costlier, toxic, and causing environment pollution. Hydrotropic solution may be a proper choice to preclude the use of organic solvents so that an attempt has been made to develop simple, accurate, novel, safe and precise spectrophotometric method for estimation of poorly-water soluble drug Glipizide. Solubility of Glipizide is increased by using tri-sodium citrate and urea solution as hydrotropic agent. There was more than 28 fold solubility enhanced in hydrotropic solution as compare with distilled water. The Glipizide shows the maximum absorbance at 274.5 nm. At this wavelength hydrotropic agent and other tablet excipients do not shows any significant interference in the spectrophotometric assay. The developed method was found to be linear in the range of 5-40 μg/ml with correlation coefficient (r2) of 0.999. The mean percent label claims of tablets of Glipizide in marketed formulation estimated by the proposed method were found to be 99.73±0.004 respectively. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. As hydrotropic agent used in the proposed method so this method is eco-friendly and it can be used in routine quantitative analysis of drug in bulk drug and dosage form in industries
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