Semen analysis: corelation with relavent to patholgoy and microbiology parameters in male infertility

The study was aimed to assess microbiological quality of male patients with infertility at Vivek Laboratories, Nagercoil. Infertility is a matter of concern in India because of its social problems. Seventy semen specimens were collected from males with infertility. The seminal fluids were diluted with sterile saline, centrifuged and cultured on Nutrient agar, Blood agar, Chocolate agar and MacConkey agar then incubated aerobically and in 5% CO2 at 37°C for 24 hours for the isolation of pathogenic microorganism. Isolates were identified based on Gram’s staining and biochemical tests. Escherichia coli was the most common isolate followed by Staphylococcus aureus, Pseudomonas aeruginosa, Proteus sps and Enterococcus sps. Of the total samples, 63% were infected and significantly affected the semen parameters. The semen motility rate was reduced significantly in samples infected with pathogenic microorganisms.