The efficacy of rhizospheric fungus Aspergillus sp., (NBP-53) was investigated for plant growth promotion and also tested for its effect on inducing resistance against anthracnose disease of chilli. The result indicated that seed treatment with isolated NBP-53 PGPF showed enhanced seed germination (66%) and seedling vigor (522.97) in comparison to that of control. The treatment also enhanced mean shoot and root length, fresh shoot and root weight of chilli plants under greenhouse conditions. In addition, it also offers considerable protection (58%) against anthracnose disease-causing pathogen Colletotrichum capsici. However, the maximum effect of enhanced seedling growth and protection was observed in positive control (Dithane M45) under both in vitro and greenhouse conditions. Therefore the study validated the use of beneficial microbes like PGPFs as a bio control agents in sustainable management of the plant diseases.