In the present research, attempts were made to isolate biosurfactant producing bacteria using mineral salt media with diesel as sole carbon source and to analyze its antimicrobial activity against non- pathogenic strains. Among 16 different strains, the most efficient strain namely Strain 21 has remarkably reduced surface tension up to 28.7 mN/m and emulsification index 74.04%. Biosurfactant was extracted using chloroform methanol in 2:1 ratio and purified using column chromatography. The composition of biosurfactant was confirmed as lipopeptide by biochemical and FTIR analysis. Based on the biochemical and molecular characterization, the strain was identified as Pseudomonas aeruginosa ARCH1802. Antimicrobial effect of biosurfactant synthesized by Pseudomonas aeruginosa ARCH1802 was confirmed by measuring their zone of inhibition against four non-pathogenic strains.
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2022: 6.012
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