Rp-hplc method development and validation for the simultaneous estimation of grazoprevir and elbasvir in bulk and pharmaceutical dosage form

A simple, Accurate, precise method was developed for the simultaneous estimation of the Grazoprevir and Elbasvir in Tablet dosage form. Chromatogram was run through Kromosil (250mm 4.6mm, 5µ). Mobile phase containing 0.01n Kh2po4 Buffer and Acetonitrile and methanol in the ratio of 45:55 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength for Grazoprevir and Elbasvir was 260nm. Retention time of Grazoprevir and Elbasvir were found to be 2.938min and 2.100min. %RSD of the Grazoprevir and Elbasvir were and found to be 0.9and 0.5 respectively. %Recover was Obtained as 99.68% and 99.34% for Grazoprevir and Elbasvir. LOD, LOQ values were obtained from regression equations of Grazoprevir and Elbasvir were 0.26ppm, 0.78ppm and 0.18ppm, 0.55ppm respectively. Regression equation of Grazoprevir is y = 43118x+27765, and of Elbasvir is y =31499 + 35670.Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries