Recent development of fluorogenic probes to detect histone deacetylase (hdac) enzyme activity in various approaches

Histone deacetylases (HDACs) are important enzymatic regulators of many cellular processes such as cell cycle, gene expression and tumorigenesis. HDACs enzymes are the major targets of drug development for diseases such as neurological diseases, metabolic diseases and cancer. Due to have attracting attention of HDACs, the detection of HDAC activities is key factor to medical sciences as well as basic biological research. Detection by fluorescence techniques is highly attractive due to its high sensitivity and real-time detection approach in a non-destructive way. Recently, various research have been made to develop the investigation of deacetylation of HDAC substrates based on intramolecular transesterification reaction, aggregation induced emission, intramolecular imine bond formation etc. This review article covers the design of fluorogenic probes and mechanistic behaviour for detection of HDAC activity.