Production of l-asparaginase from rhizosphere soil fungus aspergillus tamari

L-asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) as of late has been considered as a promising chemotherapeutic specialist against Acute Lymphoblastic leukemia specifically. Its generation, from the contagious sources is currently been broadly considered. Therapeutic use of L-asparaginase from announced sources is restricted because of the immunological reactions prompting to allergies on long term use, unfavorably susceptible responses, hypersensitivity and occurrences of unconstrained resistance of tumor cells. The present investigation aims at the production of extracellular L-asparaginase from fungal sources isolated from rhizospheric soil. The potent producer of L-asparaginase was characterized as Aspergillus tamarii BBKNM3 based on the morphology and molecular characterization. The optimization of production of L-asparaginase was studied using different agro wastes with isolated fungal strains by solid state fermentation. Isolates grown in the medium supplemented with 1 % L-asparagine as sole nitrogen source yielded the higher productivity of enzyme after72 hrs of incubation period, at 37 oC and pH 7. Additionally, 65% of initial moisture content, glucose (0.5% w/v) and ammonium sulphate (0.5% w/v) was found to be optimum. The rationale behind studying fungal sources is that they are potential sources of novel drugs. Besides, non-immunogenicity is the added advantage of fungal L-asparaginase over Bacterial L-asparaginase. Hence the present investigation aims at the isolation and characterization of potent fungal producers of L-asparaginase.