The aim of the present study was to evaluate the total phenolic content, antioxidant activity and antiproliferative activity of crude extracts of Nyctanthes arbortristis. The extracts were prepared by three experimental conditions (25 °C, elevated temperature and ultrasound waves) and four solvent systems [Aqueous, Methanol, Water: Methanol (1:1) and Chloroform: Methanol (1:1)]. For screening of antiproliferative activity, a fission yeast Schizosaccharomyces pombe was used as a model. MTT assay was used for estimating the cell viability and the results were expressed as % growth inhibition. The highest phenolic content was estimated in the methanol and chloroform: methanol extracts. The antioxidant capacities measured using DPPH scavenging activity and phosphomolybdenum assay showed that the methanol and chloroform: methanol extracts had strong antioxidant capacity. Based on the MTT assay performed on yeast cells, Water: Methanol, Chloroform: Methanol and Methanol extracts showed higher antiproliferative activity than the positive control. The results of the experiments show that decrease in the cell viability was in a dose dependent manner. In conclusion, this study has indicated that Nyctanthes arbortristis extracts possess strong antioxidant and antiproliferative activity. Furthermore, it could act as a potential source for identifying new molecules with chemotherapeutic potential against cancer.
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