Triphaladi varti is an Anubhuta Yoga of herbo- mineral ayurvedic formulation used as Yoni varti (vaginal suppository) in the case of Slaishmiki Yonivyapath (vulvo vagina candidiasis) and other vaginal infections. The present work was carried out to standardize the finished product of Triphaladi Yonivarti. The ingredients of Triphaladi Yonivarti are Haritaki, Vibhitaki, Amalaki, Haridra, Nimba and Tankana. There has been an increase in demand for the Phyto-pharmaceutical products of Ayurveda so a new pharmaceutical preparation in the form of Triphaladi Yonivarti was tried to be established in the management of Shailshmiki Yonivyapath. Drug was analyzed for the following parameters like physic-chemical (loss on drying, total ash, acid insoluble ash, pH, disintegration) and High performance thin layer chromatography (HPTLC). On the basis of observations and experimental results, the study may be used as standard protocol in the further quality control researches on Triphaladi Yonivarti.