Pesticidial origin of botanical extracts with incidence of tukra mulberry on histopathology of silkworm, (bombyx mori l.)

Present study was conducted to find out the effect of various botanical extract on the tissue, cellular and sub cellular level and histopathology of silkworm, findings of the present study gives useful data concerning the changes in the insect. Three plants extract viz Azadirachta indica, Ocimum sanctum and Parthenium hysterophorus were used as experimental while untreated leaves consider as control. These botanicals were sprayed on the tukra (Pink mealy bug) infected mulberry leaves and fed to silkworm. Findings of the study suggested no change in the midgut of the silkworm feed on the botanical sprayed leaves and it was with normal columnar epithelium had striated border (microvillus) covered by the peritrophic membrane. While epithelial cells slightly sloughed midgut was reported in the silkworm fed on the tukra leaves. The outer layers of the nucleolus were reported somewhat hypertrophied and cytoplasm was reported vacuolate with mild degeneration of cell in silkworm fed on the tukra infected leaves. Silkworm fed leaves revealed almost similar changes to that of normal and there was no change in botanical sprayed fed larvae. The impact in tissue of the silkworm when fed with normal and crude botanical extracts against mealy bugs shows normalcy, but in the tukra mulberry leaves fed by silkworms the tissues shows slight degenerative with nutritional impact upon them.