In the last decade, a special emphasis has been focused on the design and performance of surgical procedures for periodontal regeneration. Specific surgical approaches have been proposed to preserve the soft tissues and to reach a stable primary closure of the wound in order to seal the area of regeneration from the oral environment. We report two cases showing isolated bone defects in which minimally invasive periodontal surgical technique was carried out by using small incisions with minimal refection of flap, in which a periodontal regeneration was attempted using regenerative material. A brief review on minimally invasive dentistry and its benefits has been discussed.