Oxidative stress in periodontitis in haryana

Background: Periodontitis is a chronic inflammatory disease associated with gram-negative bacteria characterized by connective tissue and alveolar bone destruction. Though many studies have revealed the alterations in the salivary NO and MDA concentrations, desperately only few studies have reported simultaneous estimation of the serum NO and MDA levels. Therefore present study was planned to estimate the level of NO and MDA in patients of periodontitis in population of Haryana. Methods: A total of 50 subjects were included in the study. Out of these, 25 were healthy controls and 25 were periodontitis patients. The NO level (measured as nitrite-plus-nitrate (NO(x)) concentration) was estimated by Griess reagent method. Serum MDA level was estimated by thiobarbituric acid (TBA) reaction. Results: Patients in periodontitis group showed a significant increase in serum MDA levels and NO levels when compared to controls (p < 0.05 and < 0.001 respectively). As per Pearson’s correlation coefficient, serum NO levels were found to be positively correlated with serum MDA levels in periodontitis patients but the correlation was not significant statistically. Conclusion: Patients with periodontitis show higher systemic oxidative stress and inflammation as compared to healthy controls. NO and MDA can be considered as a biological marker, the presence of which in serum can partially determine the extent of periodontitis. Finally the use of NOS inhibitors and antioxidants can prove beneficial in limiting the progress of disease.