Background: Oral Squamous Cell Carcinoma (OSCC) is the sixth most common cancer worldwide. OSCC will develop from dysplastic oral mucosal lesions if an early diagnosis has not been made and treatment rendered. Feulgen stain is a reliable and specific histochemical method for nuclear DNA. Morphometric analysis is simple, fast and reliable when using the computer assisted area measurement and it is felt that this technique might be an adjunct to the diagnostic possibilities for determining tumor behavior.
Aim & Objective:
To study and compare the nuclear changes in epithelial cells of Oral Leukoplakia (OL) and OSCC with normal buccal mucosa (NBM) using computer aided image analysis in tissue section.
Materials & Method:
The Study group consisted of 60 cases;30 of OL and 30 cases of OSCC. The control group consisted of 10 cases of NBM. The specimen was subjected to Feulgen Stain and H&E stain. The images were captured using (Olympus E331 SLR) Digital Camera by attaching to Olympus research microscope (BX-51). All measurements were done using the measuring tools of the image analyzer software.
Statistical Analysis: Using Chi-Square Test and ANOVA and Correlation were assessed using Pearson’s Correlation test.
Results: Our results were significant for the morphometric parameter. The values of nuclear perimeter and are increased gradually from NBM to OL to SCC.
Impact Factor
2022: 6.012
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