Nanostructured Zinc oxide (ZnO) and Titanium dioxide nanoparticles (TiO2 NPs) were synthesized successfully using the Sol-gel method. The synthesized ZnO and TiO2 Nps were investigated by means of XRD, FT-IR, DLS, FE-SEM and HR-SEM techniques. XRD analysis confirmed the crystalline nature of ZnO and TiO2 nanoparticles with hexagonal Wurtzite and tetragonal structure, respectively. The calculated crystallite size matched with the HR-SEM data. Using Dynamic light scattering (DLS) technique the size distribution of the average particles histogram of ZnO and TiO2 Nps were determined. FT-IR analysis established the fundamental structure of ZnO and TiO2 NPs. The morphology of the NPs was confirmed by the FE-SEM analysis. HR-SEM analysis reveals the spherical structure of ZnO and TiO2 NPs with the average particle size ranging from 20-40 nm. The synthesized ZnO and TiO2 NPs could be utilized for a variety of interesting applications. The synthesized ZnO NPs and TiO2 NPs studied the antibacterial activity.