A simple, accurate, rapid, precise and reproducible spectrophotometric method was developed and validated for the estimation of Brivudine in pharmaceutical dosage form. The developed UV spectrophotometric method for the estimation of Brivudine is based on the measurement of absorption at maximum wavelength 250 nm using methanol as the solvent. The stock solutions of Brivudine were prepared and subsequent suitable dilutions was prepared in methanol to obtain a standard curve. The standard solutions of Brivudine show absorption maxima at 250 nm. The drug obeyed Beer-Lambert’s law in the concentration range of 2-10 µg/ml with regression 0.9997 at 250 nm. The overall % recovery do not differ significantly from 100 % which reflects that there was no interferences from common excipients used in tablet formulation. The low %RSD indicating accuracy and reproducibility of the method. The % RSD for inter-day and intra-day was found to be 0.3466 and 0.49 respectively which is <2% hence proved that the method is precise. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, limit of quantitation as per ICH guidelines. The developed method can be adopted for the routine analysis of Brivudine in tablet dosage form as well bulk dosage form.
Impact Factor
2022: 6.012
This work is licensed under a