This study aims to the enumeration of the colony forming units was done for the quantitative population analysis of different spoiled food samples. Highest count of 1.21×102 cfu/ml was observed in the case of pineapple sample with lowest count observed at 0.28×102 cfu/ml for bread sample. The isolates obtained from food samples were then stained and also identified based on their biochemical properties. The media used for enumeration were Plate CountAgar, Nutrient Agar and MacConkey Agar media for the count of total aerobic and anaerobic microbes. The targeted species were Listeria monocytogenes, Coliform bacteria, Escherichia coli, Yeast & Molds, Lactobacillus, Salmonella typhi, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, as these species have their role in causing spoilage of food samples. The identification was done based on biochemical tests including catalase test, indole test, sugar fermentation test, urease test, oxidase test, citrate utilization test. A total of 23 bacterial, 3 yeast, and 7 mold isolates were isolated from the food samples. These isolates were then classified by categorizing them into 13 bacterial species, 3 yeast species, and 4 mold species based on their phenotypic characteristics.
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2022: 6.012
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