Isolated strain AP13 was good source of alkaline protease it was found to be It was found to be gram positive. Catalase, oxidase and citrate tests were positive. Test such as methyl red, indole and VP was found to be negative. It showed clear zone on casein hydrolyzed media. The strain AP13 produced amount of proteolytic enzyme of 498.345 units/mg after the incubation of 24hrs at 37 ͦ C. As the time of incubation increased the proteolytic activity got decreased
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