Isolation and Identification of FTase producing fungi from different sources

The aim of this research was to identify microorganisms capable of synthesizing Fructosyltransferase (FTase) enzyme from honey and canned sugar syrups. The microbial isolates were cultivated on Potato Dextrose Agar (PDA) supplemented with 20% sucrose to promote the growth of osmophilic fungi. Following isolation, the bacteria were tested for FTase production by watching filamentous fungal growth. The Vogel’s medium was utilized to maximize enzyme production, with various carbon and nitrogen sources added. The inoculum preparation and culture conditions were carefully tuned to maximize enzyme output. To characterize the FTase activity, the ideal pH and temperature conditions for enzyme action were determined using the glucose oxidase-peroxidase method. The effects of various carbon and nitrogen sources were investigated, including pure sugars and complex substrates such as pineapple peel.Furthermore, the time course of FTase production was studied to establish the best production period. The enzyme activity was evaluated by measuring invertase and FTase activity, which release reducing sugars from sucrose and glucose production, respectively. The results showed that some carbon and nitrogen sources, specifically pineapple peel and ammonium chloride, dramatically increased FTase synthesis. The maximum enzyme activity was obtained under particular culture conditions, such as a controlled pH of 6.0 and a temperature of 28°C. These findings offer important insights into enhancing FTase production, which has enormous industrial potential for use in biotransformation, food processing, and other industries.